Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11. Single-cell transcriptomics of the human endocrine pancreas. Single-cell transcriptome profiling of human pancreatic islets in health and type 2 diabetes. Single-cell resolution analysis of the human pancreatic ductal progenitor cell niche. A single-cell transcriptome atlas of the human pancreas. A single-cell transcriptomic map of the human and mouse pancreas reveals inter- and intra-cell population structure. Single-cell analysis of human pancreas reveals transcriptional signatures of aging and somatic mutation patterns. The Tabula Muris Consortium Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris. Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Modelling plasticity and dysplasia of pancreatic ductal organoids derived from human pluripotent stem cells. Development of the human pancreas from foregut to endocrine commitment.

Nkењ† transcription factors and Ptf1a function as antagonistic lineage determinants in multipotent pancreatic progenitors. SOX9+/PTF1A+ cells define the tip progenitor cells of the human fetal pancreas of the second trimester.

A multipotent progenitor domain guides pancreatic organogenesis. Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell- and patient-derived tumor organoids. Human pluripotent stem cell-derived acinar/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling. Model organoids provide new research opportunities for ductal pancreatic cancer. Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids. Organoid profiling identifies common responders to chemotherapy in pancreatic cancer. Pancreatic cancer-derived organoids-a disease modeling tool to predict drug response. Cancer modeling meets human organoid technology. Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis. Organoid models of human and mouse ductal pancreatic cancer. Cell of origin affects tumour development and phenotype in pancreatic ductal adenocarcinoma. p53 mutations cooperate with oncogenic Kras to promote adenocarcinoma from pancreatic ductal cells. Duct- and acinar-derived pancreatic ductal adenocarcinomas show distinct tumor progression and marker expression. Stem cell-derived organoids to model gastrointestinal facets of cystic fibrosis. Molecular mechanism of pancreatic and salivary gland fluid and HCO 3 secretion. The PDLO microsystem could be used to establish patient-specific pancreatic duct models. We also used the chip to uncover ductal markers relevant to pancreatic carcinogenesis, and to establish PDLO co-cultures with stellate cells, which allowed for the study of epithelialвЂ“mesenchymal signalling. PDLO subpopulations expressed either mucins or the cystic fibrosis transmembrane conductance regulator, and resembled human adult duct cells. Using time-resolved single-cell transcriptional profiling and immunofluorescence imaging of the forming PDLOs, we identified differentiation routes from pancreatic progenitors through ductal intermediates to two types of mature duct-like cells and a few non-ductal cell types. Here we report the single-cell characterization of the differentiation of pancreatic duct-like organoids (PDLOs) from human induced pluripotent stem cells (hiPSCs) on a microwell chip that facilitates the uniform aggregation and chemical induction of hiPSC-derived pancreatic progenitors. Creating in vitro models of diseases of the pancreatic ductal compartment requires a comprehensive understanding of the developmental trajectories of pancreas-specific cell types.

Ab cam duct